erg7 deficient yeast mutant  (ATCC)

Journal: PLOS One

Article Title: A rare ORAI1 missense variant associates with risk of vascular diseases in White British adults

doi: 10.1371/journal.pone.0337519

Figure Lengend Snippet: Functional characterisation of the ORAI1 S218G mutation in the Ca 2 + addback assay. (A,B) Representative fluorescence over time graph of thapsigargin (TG) induced store operated Ca 2+ entry. Data are baseline corrected. TG is added before recording. Ca 2+ addback time = 30 seconds (s). Data presented as mean ± SEM (n = 4). (B) Quantification of peak Ca 2+ entry in wild type HEK293 cells and cells with the S218G variant. Data presented as mean ± SEM (N = 16). (C) IC 50 curve for inhibition of ORAI1 by JPIII in wild type HEK293 cells and cells with the S218G variant (n = 3). ORAI1 S218G HEK293 cells (green), WT HEK293 cells (black) and control cells not exposed to TG; ORAI1 S218G HEK293 cells (light green), WT HEK293 cells (grey). Statistical significance using one-way ANOVA with Tukey’s post hoc test ns = not significant, **** P < 0·0001.

Article Snippet: HEK293 cells (ATCC, Teddington, UK) and HEK293 c.A658G mutant cells purchased from Ubigene Biosciences (Guangzhou, China) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 10% v/v heat-inactivated foetal bovine serum (FBS), and 1% penicillin/streptomycin at 37°C in a humidified incubator at 5% CO 2 .

Techniques: Functional Assay, Mutagenesis, Fluorescence, Variant Assay, Inhibition, Control

Journal: bioRxiv

Article Title: Arachidonic acid modulates fluconazole-induced responses in Candida albicans biofilms

doi: 10.1101/2025.11.25.690624

Figure Lengend Snippet: Role of arachidonic acid (AA) in response to fluconazole-induced oxidative stress. A) Effect of fluconazole on biofilm formation of selected heterozygous mutants. B) Effect of fluconazole on PST1 heterozygous mutants ( pst1Δ/PST1 ) and the reversals in the presence of butylated hydroxytoluene (BHT). C) Biomass of Candida albicans cdr1 Δ/Δ in the presence of 0.1 mM arachidonic acid (AA) and antioxidants. Percentage biofilm biomass after 6 h of cdr1 Δ/Δ in the presence of 0.1 mM arachidonic acid compared to ethanol control (0.076%). In addition, 0.5 mM α-tocopherol and 0.045 mM butylated hydroxytoluene (BHT) were added, respectively. Values are the mean of three independent experiments, and the standard deviation is indicated by the error bars.*Significantly different from cdr1Δ/Δ with only arachidonic acid (* P < 0.05).

Article Snippet: Candida albicans heterozygous mutants corresponding to selected genes, exhibiting changes in the levels of differential regulation in the presence of fluconazole and arachidonic acid, were selected from a double-barcoded library of heterozygous C. albicans mutants (version 2013A) supplied by Merck Sharp & Dohme Corp ( ) ( Roemer et al ., 2003 ).

Techniques: Control, Standard Deviation